INVITRO ANTI-CANCER STUDY OF HERBAL EXTRACT ON COLON CARCINOMA CELLS

Abstract: Introduction: Plant products are important and attractive in field of scientific investigation are considered a rich source of pharmacologically active molecules and for new drug development. The current research aimed to assess anticancer effect of herbal extract on colo-205 cancer cell lines. . Here, we investigated anti-cancer effects of three herbal drug extracts-Ixora coccinea family Rubiaceae flower and Piper longum family Piperaceae, vitis vinifere family vitiaceae-Grape seed (GSE) root extract to treat against colon cancer . Method: Several qualitative phytochemical tests were performed on Ixora coccinea,Piper longum and vitis vinifere by using standard phytochemical procedures to assessment of cytotoxic MTT assay using standard biochemical methods in Colo-205 cells .With supplement of 1% penicillin,of 10% inactivated Fetal Bovine Serum,cells were cultured in DMEM , using 5% CO2 at humidified atmosphere(37oC). Results:The percentage inhibition(IC50 value) of Piper longum (0–640 µg/ml),vitis vinifera (0–640 µg/ml) and Ixora coccinea (0–640 µg/ml) treatment of cells were showed following result, 57% (636 µg/ml), 52% (366µg/ml) and 58% (244µg/ml) growth inhibition respectively. Decreased cell proliferation in time-dependent manner was observed.